Cirulli F, Alleva E
Behavioral Pathophysiology Section, Istituto Superiore di Sanità, Rome, Italy.
Neurotoxicology. 1994 Winter;15(4):819-25.
In this study we tested the effects of repeated administrations of Epidermal Growth Factor (EGF) and Transforming Growth Factor-alpha (TGF-alpha) on mouse pups' neurobehavioral development. Each subject was injected subcutaneously with either EGF or TGF-alpha on postnatal days 2, 4, 6, 8, and 10. Pups treated with these two peptides showed accelerated eyelid opening and eruption of the lower incisors when compared to Cytochrome c-injected control littermates. EGF, but not TGF-alpha, resulted in a slight body growth retardation. When scored for a number of neurobehavioral parameters, EGF pups showed a delayed appearance of the righting reflex. Also, EGF-treated pups exhibited greater ultrasonic vocalization calling rates than controls when tested on postnatal day 7. Overall, TGF-alpha administration resulted in minor effects, when compared with EGF treatment, probably as a result of the lower dose administered (EGF: 3.5 mg/kg vs TGF-alpha: 1 mg/kg). TGF-alpha affected pups' eyelid opening and incisor eruption, similarly to EGF, but seemed to exert an opposite effect on some neurobehavioral scores, in line with what was already reported for Nerve Growth Factor (NGF) (Calamandrei and Alleva, 1989). These results confirm the role played by polypeptide growth factors on mammalian physical and neurobehavioral development and suggest that TGF-alpha might affect mouse brain development in a similar fashion as NGF.
在本研究中，我们测试了重复给予表皮生长因子（EGF）和转化生长因子-α（TGF-α）对幼鼠神经行为发育的影响。在出生后第2、4、6、8和10天，给每个实验对象皮下注射EGF或TGF-α。与注射细胞色素c的同窝对照幼崽相比，用这两种肽处理的幼崽眼睑张开和下切牙萌出加速。EGF导致轻微的身体生长迟缓，但TGF-α没有。在对一些神经行为参数进行评分时，注射EGF的幼崽翻正反射出现延迟。此外，在出生后第7天进行测试时，注射EGF的幼崽发出的超声波叫声频率高于对照组。总体而言，与EGF处理相比，TGF-α的给药产生的影响较小，这可能是由于给药剂量较低（EGF：3.5mg/kg vs TGF-α：1mg/kg）。TGF-α对幼崽的眼睑张开和切牙萌出的影响与EGF相似，但似乎对一些神经行为评分产生相反的影响，这与神经生长因子（NGF）的报道一致（卡拉曼德雷和阿莱瓦，1989年）。这些结果证实了多肽生长因子在哺乳动物身体和神经行为发育中所起的作用，并表明TGF-α可能以与NGF类似的方式影响小鼠大脑发育。
