Gheri G, Bryk S G, Sgambati E
Department of Human Anatomy and Histology, University of Florence, Policlinico di Careggi, Italy.
Acta Histochem. 1994 Dec;96(4):387-97. doi: 10.1016/S0065-1281(11)80023-1.
Using seven horseradish peroxidase-conjugated lectins we studied the distribution of glycoconjugate sugar residues in the lining and glandular epithelium of the gizzard in chick embryos from day 7 to 21 of incubation. At the outer layer of the lining epithelium all investigated sugar residues were present from day 7 onwards, except alpha-L-fucose, which was detected later. From day 9 some cells, which contained granules characterised by D-galactose-(beta 1--->3)-N-acetyl-D-galactosamine and N-acetyl-D-glucosamine, were observed in the inner part of the lining epithelium. Afterwards, although in different time periods, all investigated oligosaccharides were detected in intercellular spaces filled with mucous material. At first, the cells of the anlage of the tubular glands appeared to be characterised by D-glucosamine and alpha-D-mannose only. Afterwards, all the investigated sugar residues were detected. At hatching, the luminal glandular secretion showed all investigated sugar residues, except alpha-L-fucose, which was typical for the early formation of the tubular glands. Our data suggest that during the development of the gizzard three time periods of mucous release can be distinguished.
我们使用七种辣根过氧化物酶偶联凝集素，研究了孵化第7天至21天鸡胚砂囊内衬和腺上皮中糖缀合物糖残基的分布。在内衬上皮的外层，从第7天起就存在所有研究的糖残基，但α-L-岩藻糖除外，它在之后才被检测到。从第9天开始，在内衬上皮内部观察到一些细胞，这些细胞含有以D-半乳糖-（β1--->3）-N-乙酰-D-半乳糖胺和N-乙酰-D-葡萄糖胺为特征的颗粒。此后，尽管在不同时间段，在充满粘液物质的细胞间隙中检测到了所有研究的寡糖。起初，管状腺原基的细胞似乎仅以D-葡萄糖胺和α-D-甘露糖为特征。此后，检测到了所有研究的糖残基。在孵化时，腺腔分泌物显示出所有研究的糖残基，但α-L-岩藻糖除外，它是管状腺早期形成的典型特征。我们的数据表明，在砂囊发育过程中，可以区分出三个粘液释放的时间段。
