Gheri G, Bryk S G, Petrelli V
Department of Human Anatomy and Histology, University of Florence, Italy.
Histochemistry. 1990;95(1):63-71. doi: 10.1007/BF00737229.
Fragments of mesonephros were taken from chick embryos and studied from the 4th to the 21st day of incubation. A battery of seven different horseradish peroxidase-labelled lectins was used to study the distribution of carbohydrate residues in glycoconjugates along the mesonephric nephron during the period of excretory activity and the period of involution. ConA and WGA reacted at every site of the nephron thus showing the ubiquitous presence of alpha-D-mannose and N-acetyl-D-glucosamine. SBA was a good marker of the proximal tubule. Other lectins, such as PNA and LTA, reacted only for a short time at some sites during the considered period of incubation. The presence of sialic acid was detected in the podocytes, capillary wall and mesangial cells. From the 10th-11th day of incubation changes were noted in the proximal tubule as shown by PNA reactivity. This may be significant as regards the exact stage of incubation during which the involution of mesonephros begins.
从鸡胚中获取中肾碎片，并在孵化的第4天至第21天进行研究。使用一组七种不同的辣根过氧化物酶标记凝集素，以研究在排泄活动期和退化期沿中肾肾单位的糖缀合物中碳水化合物残基的分布。伴刀豆球蛋白A（ConA）和小麦胚凝集素（WGA）在肾单位的每个部位都有反应，从而表明α-D-甘露糖和N-乙酰-D-葡萄糖胺普遍存在。大豆凝集素（SBA）是近端小管的良好标记物。其他凝集素，如花生凝集素（PNA）和扁豆凝集素（LTA），在考虑的孵化期内仅在某些部位短时间反应。在足细胞、毛细血管壁和系膜细胞中检测到唾液酸的存在。从孵化的第10 - 11天开始，近端小管出现了如PNA反应性所示的变化。这对于中肾开始退化的确切孵化阶段可能具有重要意义。
