Watson D G, Su Q, Midgley J M, Doyle E, Morton N S
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
J Pharm Biomed Anal. 1995 Jan;13(1):27-32. doi: 10.1016/0731-7085(94)00121-h.
A sensitive method for the analysis of unconjugated morphine, codeine, normorphine and total morphine after hydrolysis of glucuronide conjugates is described. The method was applicable to 50-microliters volumes of plasma. The analytes were converted to heptafluorobutyryl (HFB) derivatives before analysis by gas chromatography-negative ion chemical ionization mass spectrometry. Morphine and codeine were quantified against their [2H3]-isotopomers. Linearity, precision and accuracy were quite acceptable (in the 10(-10)-10(-9) g range), and the absolute limits of detection were < 1 pg.
本文描述了一种用于分析葡萄糖醛酸共轭物水解后游离吗啡、可待因、去甲吗啡和总吗啡的灵敏方法。该方法适用于50微升血浆样本。分析物在通过气相色谱 - 负离子化学电离质谱进行分析之前，先转化为七氟丁酰（HFB）衍生物。吗啡和可待因通过其[2H3] - 同位素异构体进行定量。线性、精密度和准确度相当令人满意（在10(-10)-10(-9)克范围内），绝对检测限<1皮克。
