Tootell R B, Taylor J B
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Cereb Cortex. 1995 Jan-Feb;5(1):39-55. doi: 10.1093/cercor/5.1.39.
We stained human visual cortex for myelin, cytochrome oxidase, and the monoclonal antibody CAT-301 in an attempt to demonstrate and map MT (V5) and other visual cortical areas in humans. Both flattened and unflattened cortical tissue was examined. A likely candidate for area MT (V5), which we refer to as MT, was demonstrated using all three stains. Myelin and CAT-301 labels for MT were demonstrated to be coincident by comparing results from the two stains in adjacent sections. In all three stains, MT was an oval area approximately 1.2 x 2.0 cm, located 5-6 cm anterior and dorsal to the foveal V1-V2 border. The position and size of MT as defined by the present anatomy are consistent with MT (V5) as defined by functional measures in humans. In addition, flattened cortical tissue stained for cytochrome oxidase revealed a distinctive staining topography in several cortical areas, including areas V1, V2, MT, PX, and VX. Similar studies in flattened cortex of macaque and green monkeys demonstrated distinctive dark cytochrome oxidase staining in MT, PX, MTc, and V3.
我们对人类视觉皮层进行了髓磷脂、细胞色素氧化酶和单克隆抗体CAT - 301染色，试图在人类中显示并绘制MT（V5）和其他视觉皮层区域。我们检查了扁平及未扁平的皮层组织。利用这三种染色方法，我们发现了一个可能是MT区域（V5）的区域，我们将其称为MT。通过比较相邻切片上两种染色的结果，证实MT的髓磷脂和CAT - 301标记是重合的。在所有三种染色中，MT是一个椭圆形区域，约1.2×2.0厘米，位于中央凹V1 - V2边界前方和背侧5 - 6厘米处。根据目前的解剖学定义，MT的位置和大小与通过人类功能测量定义的MT（V5）一致。此外，对细胞色素氧化酶染色的扁平皮层组织显示，在包括V1、V2、MT、PX和VX等几个皮层区域有独特的染色地形图。在猕猴和绿猴的扁平皮层中进行的类似研究表明，MT、PX、MTc和V3中有独特的深色细胞色素氧化酶染色。
