Lowe K C, Furmidge B A, Thomas S
Department of Life Science, University of Nottingham, U.K.
Artif Cells Blood Substit Immobil Biotechnol. 1995;23(1):135-9. doi: 10.3109/10731199509117673.
The effects of incubating blood from mice, rats, rabbits or hamsters with either a commercial grade or a silica-purified fraction of Pluronic F-68 or Pluronic F-38 have been studied. Incubation of blood with up to 4.0% (w/v) of commercial or purified Pluronic F-68 produced no detectable haemolysis (< 0.1%). Haemolysis did occur with concentrations of commercial Pluronic F-68 above 4.0% (w/v). This was maximal with rat blood incubated with 10.0% (w/v) Pluronic F-68, where the mean haemolysis was 4.7 +/- 1.5%; the mean haemolysis in rat blood was reduced to 0.5 +/- 0.3% (P < 0.05) following incubation with the purified Pluronic F-68 fraction. Neither commercial grade or purified Pluronic F-38 produced any significant haemolysis when incubated with rat or hamster blood. Incubation of rabbit blood with 10.0% (w/v) commercial Pluronic F-38 produced only 0.5% haemolysis.
研究了将小鼠、大鼠、兔子或仓鼠的血液与商业级或经硅胶纯化的普朗尼克F - 68或普朗尼克F - 38组分一起孵育的效果。用高达4.0%（w/v）的商业级或纯化的普朗尼克F - 68孵育血液未检测到溶血（<0.1%）。当商业级普朗尼克F - 68的浓度高于4.0%（w/v）时确实发生了溶血。在用10.0%（w/v）普朗尼克F - 68孵育大鼠血液时溶血最为严重，此时平均溶血率为4.7±1.5%；在用纯化的普朗尼克F - 68组分孵育后，大鼠血液中的平均溶血率降至0.5±0.3%（P<0.05）。当商业级或纯化的普朗尼克F - 38与大鼠或仓鼠血液一起孵育时，均未产生任何显著的溶血现象。用10.0%（w/v）商业级普朗尼克F - 38孵育兔血仅产生0.5%的溶血。
