Xu D, Wu T, Wu X, Zhang Y, Chen Y
Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai, China.
Chin J Biotechnol. 1995;11(2):101-7.
The potential toxicity and optimal concentrations of different protective agents such as pluronic F-68, methylcellulose (MC), CMC, and GPE on the in vitro growth of murine hybridoma 2F7 cells that secrete monoclonal antibody against small-cell lung cancer were studied. The effect on the rate of glucose utilization of adding protective agents was investigated. The protective effects of different concentrations of the protective agents at high agitation speed were also observed. It showed that 0.05% to 0.10% (w/v) of pluronic F-68 and 0.10% to 0.20% (w/v) of MC could protect hybridoma cells from shear stress at high agitation speed. Adding pluronic F-68 could increase glucose utilization rate, but increased the ammonia production rate. Although CMC did not affect 2F7 cell growth at a concentration less than 0.10% (w/v), it exhibited no protective property. GPE could lyze hybridoma cells. In a 1.5-L CelliGen bioreactor, when the pluronic F-68 concentration was 0.10% (w/v) in the medium and agitation speed was 70 r/min, the hybridoma cells could grow normally.
研究了不同保护剂如普朗尼克F - 68、甲基纤维素（MC）、羧甲基纤维素（CMC）和甘油磷酸酯（GPE）对分泌抗小细胞肺癌单克隆抗体的小鼠杂交瘤2F7细胞体外生长的潜在毒性和最佳浓度。研究了添加保护剂对葡萄糖利用率的影响。还观察了不同浓度保护剂在高搅拌速度下的保护作用。结果表明，0.05%至0.10%（w/v）的普朗尼克F - 68和0.10%至0.20%（w/v）的MC可在高搅拌速度下保护杂交瘤细胞免受剪切应力。添加普朗尼克F - 68可提高葡萄糖利用率，但会增加氨的产生率。尽管CMC在浓度低于0.10%（w/v）时不影响2F7细胞生长，但它没有保护作用。GPE可裂解杂交瘤细胞。在1.5升CelliGen生物反应器中，当培养基中普朗尼克F - 68浓度为0.10%（w/v）且搅拌速度为70转/分钟时，杂交瘤细胞可正常生长。
