Shephard G S, Thiel P G, Sydenham E W
Programme on Mycotoxins and Experimental Carcinogenesis, Medical Research Council, Tygerberg, South Africa.
J Chromatogr A. 1995 Feb 10;692(1-2):39-43. doi: 10.1016/0021-9673(94)00559-r.
The fungus Fusarium moniliforme produces a group of mycotoxins, the fumonisins, of which the most abundant are fumonisins B1 (FB1) and B2 (FB2). Previously developed analytical methods for the determination of FB1 in physiological samples have been modified for the determination of FB2 by the use of less polar extraction solvents. Plasma and urine extracts were purified on strong anion-exchange solid-phase extraction cartridges and fecal extracts on reversed-phase (C18) cartridges. FB2 in purified extracts was determined by reversed-phase HPLC with fluorescence detection using performed o-phthaldialdehyde derivatives. These methods were reproducible (R.S.D. of less than 6%) with recoveries greater than 85%. In a short preliminary study, they have been applied to the determination of the fate of FB2 dosed to rats by gavage. Of the dose given to the animals, over 90% was recovered unmetabolised in the feces within 48 h.
镰刀菌产生一组霉菌毒素，即伏马毒素，其中含量最高的是伏马毒素B1（FB1）和B2（FB2）。以前开发的用于测定生理样品中FB1的分析方法已通过使用极性较小的萃取溶剂进行修改，以测定FB2。血浆和尿液提取物在强阴离子交换固相萃取柱上进行纯化，粪便提取物在反相（C18）柱上进行纯化。纯化提取物中的FB2通过反相高效液相色谱法（HPLC）进行测定，使用邻苯二甲醛衍生化后进行荧光检测。这些方法具有可重复性（相对标准偏差小于6%），回收率大于85%。在一项简短的初步研究中，它们已应用于测定经口灌胃给予大鼠的FB2的代谢情况。给予动物的剂量中，超过90%在48小时内未代谢而在粪便中回收。
