Lemaire P, Garrett N, Gurdon J B
Wellcome Trust/Cancer Research Campaign Institute, England.
Cell. 1995 Apr 7;81(1):85-94. doi: 10.1016/0092-8674(95)90373-9.
Using an expression cloning strategy that relies on a functional assay, we have cloned a novel Xenopus homeobox-containing gene, Siamois. Embryos injected in a ventral-vegetal blastomere with as little as 5 pg of Siamois mRNA develop a complete secondary axis, but the progeny of the injected cells do not participate in the secondary axis formation. In normal development, Siamois mRNA is first detected shortly after the midblastula transition, which is earlier than mRNAs for goosecoid or Xbrachyury, and is present most abundantly in the dorsal endoderm of early gastrulae. The activation of this gene can be obtained cell autonomously in dispersed embryo cells. These results indicate that Siamois may play an important role in the formation of the Nieuwkoop center.
利用一种依赖功能检测的表达克隆策略，我们克隆了一个新的含有爪蟾属同源异型框的基因——暹罗基因。向腹侧植物极卵裂球中注射低至5皮克的暹罗基因信使核糖核酸（mRNA）的胚胎会发育出完整的次生轴，但注射细胞的后代并不参与次生轴的形成。在正常发育过程中，暹罗基因mRNA最早在中囊胚转换后不久就能检测到，这比 goosecoid 或 Xbrachyury 的mRNA出现得更早，并且在早期原肠胚的背侧内胚层中含量最为丰富。该基因的激活可以在分散的胚胎细胞中自主实现。这些结果表明，暹罗基因可能在Nieuwkoop中心的形成中发挥重要作用。
