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[湖北钉螺三种酶的电泳图谱分析]

[Analysis of electrophoretic patterns of three enzymes of Oncomelania hupensis].

作者信息

Wang X

机构信息

Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(4):271-4.

PMID:7720201
Abstract

Allozyme electrophoresis was carried out in starch gel to compare the electrophoretic patterns for acid phosphatase (AcP, EC 3. 1. 3.2), peroxidase (Po, EC 1.11.1.7) and esterase (Est, EC 3. 1. 1. 1). It was found in this study that isoenzymes AcP1, AcP2 and Po1, Po2 were coded by 2 loci respectively and they were monomorphic. It is possible to determine 4 loci responsible for esterase zymogram, among which isoenzyme Est3 region either consisted of a fast (Est3-F) band in the case of Oncomelania hupensis isolated from Anhui, or a slow (Est3-S) band in snails from Yunnan. The Rf values of the fast and slow bands were 0.362 +/- 0.027 and 0.340 +/- 0.036, respectively. Snails from Yunnan, at the upper reaches of the Yangtze River, are proved to be resistant to infection with Anhui-Hubei strain of Schistosoma japonicum in the mainland of China, while snails from Anhui, at the lower reaches of the Yangtze, susceptible to infection with Anhui-Hubei strain of S. japonicum.

摘要

采用淀粉凝胶进行等位酶电泳,以比较酸性磷酸酶(AcP,EC 3.1.3.2)、过氧化物酶(Po,EC 1.11.1.7)和酯酶(Est,EC 3.1.1.1)的电泳图谱。本研究发现,同工酶AcP1、AcP2和Po1、Po2分别由2个基因座编码,且均为单态性。酯酶酶谱可确定有4个基因座,其中来自安徽的湖北钉螺的同工酶Est3区域由一条快带(Est3-F)组成,而来自云南的钉螺则由一条慢带(Est3-S)组成。快带和慢带的Rf值分别为0.362±0.027和0.340±0.036。长江上游的云南钉螺被证明对中国大陆的安徽-湖北株日本血吸虫感染具有抗性,而长江下游的安徽钉螺则易感染安徽-湖北株日本血吸虫。

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