Zhang Y, Feng T, Davis G M
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(3):172-7.
Nine populations of Oncomelania, field-collected from Anhui, Shanghai, Jiangsu, Zhejiang, Jiangxi, Hunan, Hubei, Sichuan and Yunnan were studied by horizontal starch gel electrophoretic method with 24 enzyme systems (AAT, AcPH, AK, AO, APH, CK, EST, GDH, GPI, G6PD, HBD, ISDH, LAP, LDH, ME, MDH, MPI, NADD, OCT, PGM, 6PGD, SDH, SOD, XDH) analyzed. 40 loci and 117 alleles were detected in the Oncomelania. Both of GPI and PGM-I, with 7 alleles, were the most variable loci. 22 loci had more than 3 alleles each. Of 40 loci examined in the 24 isozyme systems, 14 were found to be polymorphic, the proportion of multilocus enzymes being 58.3%. Our results showed that the genetic polymorphism existing in the populations of Oncomelania in the mainland of China. PGM and MDH, were found in both the populations of Oncomelania and strains of Schistosoma japonicum in the mainland of China. The results provided a new idea for studying snails and Schistosoma. Also, we found that there might be some correlation between the polymorphic locus and the feature of the shell of Oncomelania snail.
采用水平淀粉凝胶电泳法,对采自安徽、上海、江苏、浙江、江西、湖南、湖北、四川和云南的9个钉螺种群进行了研究,分析了24种酶系统(AAT、AcPH、AK、AO、APH、CK、EST、GDH、GPI、G6PD、HBD、ISDH、LAP、LDH、ME、MDH、MPI、NADD、OCT、PGM、6PGD、SDH、SOD、XDH)。在钉螺中检测到40个基因座和117个等位基因。GPI和PGM-I均有7个等位基因,是变异最大的基因座。22个基因座每个都有3个以上的等位基因。在24种同工酶系统检测的40个基因座中,发现14个为多态性基因座,多位点酶的比例为58.3%。我们的结果表明,中国大陆钉螺种群中存在遗传多态性。PGM和MDH在中国大陆钉螺种群和日本血吸虫株中均有发现。这些结果为研究钉螺和血吸虫提供了新思路。此外,我们发现多态性基因座与钉螺壳的特征之间可能存在某种关联。