Witte K H, Auerbach J, Loss K U, Neuhaus S, Prager D
Staatlichen Veterinäruntersuchungsamt Arnsberg.
Dtsch Tierarztl Wochenschr. 1994 Dec;101(12):482-4.
Seventeen strains of porcine enteroviruses (PEV) were isolated from organs of 119 pigs with symptoms of polioencephalomyelitis submitted to the State Veterinary Institute Arnsberg/Westphalia between 1983 and 1991. 15 isolates originated from the central nervous system and 2 from organ suspensions made up of a brain-spleen-pool and a lung-spleen-lymph node-pool, respectively. Isolates were assigned to 7 PEV types which were present at the following frequencies: PEV1: 2x; PEV2: 6x; PEV4: 3x; PEV5: 1x; PEV6: 2x; PEV 12: 1x; PEV 13: 2x. Mortality rates of affected groups exhibited an age-dependent curvilinear relationship suggesting that the PEV involved possessed a rather similar low to medium grade neurovirulence, irrespective of type. Exceptions were 1 herd with 100% mortality at the age of 10-18 weeks from which PEV2 strain 6793/83 was isolated (described earlier) and a second herd with 18% mortality at the age of 14-18 weeks from which PEV types 1, 2 and 4 were recovered. Sensitivity of 5 cell lines for the isolation of PEV was compared. Rates of isolation from organ suspensions which had proved positive in any of the cell lines tested were as follows: PS-EK: 77%; IB-RS-2: 63%; ST: 56%; PK-15: 47%; BHK21 (CT): 10%.
1983年至1991年间，从119头患有脑脊髓灰质炎症状的猪的器官中分离出17株猪肠道病毒（PEV），这些猪被送往阿恩斯贝格/威斯特法伦州立兽医研究所。15株分离株来自中枢神经系统，2株分别来自由脑-脾混合液和肺-脾-淋巴结混合液制成的器官悬液。分离株被分为7种PEV类型，其出现频率如下：PEV1：2株；PEV2：6株；PEV4：3株；PEV5：1株；PEV6：2株；PEV 12：1株；PEV 13：2株。受感染猪群的死亡率呈现出年龄依赖性的曲线关系，这表明所涉及的PEV无论类型如何，都具有相当相似的低至中等程度的神经毒力。例外情况是，一个猪群在10 - 18周龄时死亡率为100%，从中分离出了PEV2毒株6793/83（先前已描述），另一个猪群在14 - 18周龄时死亡率为18%，从中分离出了PEV1、PEV2和PEV4型。比较了5种细胞系对PEV分离的敏感性。从在所测试的任何细胞系中已被证明为阳性的器官悬液中的分离率如下：PS - EK：77%；IB - RS - 2：63%；ST：56%；PK - 15：47%；BHK21（CT）：10%。
