Jupin I, Hericourt F, Benz B, Gronenborn B
Institut des Sciences Végétales, CNRS, Gif sur Yvette, France.
FEBS Lett. 1995 Apr 3;362(2):116-20. doi: 10.1016/0014-5793(95)00221-t.
Geminiviruses are plant DNA viruses replicating by a rolling circle mechanism. We have investigated the specificity of replication origin recognition of two different isolates of tomato yellow leaf curl virus (TYLCV). Here, we show that TYLCV-Sardinian and -Israeli replication proteins display a high degree of specificity for their respective origins. The DNA sequences recognized are located on the left part of the intergenic region whereas the amino-terminal 116 amino acids of the Rep protein determine the specificity of origin recognition.
双生病毒是通过滚环机制进行复制的植物DNA病毒。我们研究了番茄黄化曲叶病毒（TYLCV）两种不同分离株复制起点识别的特异性。在此，我们表明撒丁岛TYLCV和以色列TYLCV的复制蛋白对其各自的起点表现出高度特异性。被识别的DNA序列位于基因间隔区的左侧，而Rep蛋白的氨基末端116个氨基酸决定了起点识别的特异性。
