改进对同位素标记RNA中¹³C、³¹P J耦合常数的测量。
Improved measurement of 13C, 31P J coupling constants in isotopically labeled RNA.
作者信息
Legault P, Jucker F M, Pardi A
机构信息
Department of Chemistry and Biochemistry, University of Colorado at Boulder 80309-0215, USA.
出版信息
FEBS Lett. 1995 Apr 3;362(2):156-60. doi: 10.1016/0014-5793(95)00232-x.
3JCP coupling constants have been measured in a 99% 13C,15N labeled lead-dependent ribozyme, known as the leadzyme. These coupling constants were determined by analysis of the intensity of individual crosspeaks in a spin-echo difference constant time HSQC experiment. This procedure permits improved measurement of the 3JC2'P and 3JC4'P coupling constants in isotopically labeled RNA and yielded valuable information on the beta and epsilon backbone torsion angles in the leadzyme.
已在一种99% 13C、15N标记的依赖铅的核酶（称为铅酶）中测量了3JCP耦合常数。这些耦合常数是通过分析自旋回波差分恒时HSQC实验中各个交叉峰的强度来确定的。该方法能够改进对同位素标记RNA中3JC2'P和3JC4'P耦合常数的测量，并提供了关于铅酶中β和ε主链扭转角的有价值信息。
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