Schwalbe H, Marino J P, King G C, Wechselberger R, Bermel W, Griesinger C
Institut für Organische Chemie, Universität Frankfurt, Germany.
J Biomol NMR. 1994 Sep;4(5):631-44. doi: 10.1007/BF00404274.
Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles beta and epsilon. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle chi.
介绍了三个实验来确定RNA寡聚物中的一整套耦合常数。在HCCH-E.COSY实验中，可以高精度测量邻位质子-质子耦合常数。在P-FIDS-CT-HSQC实验中，测量了取决于磷酸二酯主链扭转角β和ε的邻位质子-磷和碳-磷耦合。在重聚焦HMBC实验中，测量了取决于糖苷扭转角χ的邻位碳-质子耦合。
