Johnston R C, Clarke G N, Liu D Y, Baker H W
Prince Henry's Institute of Medical Research, Clayton, University of Melbourne, Victoria, Australia.
Fertil Steril. 1995 May;63(5):1071-6.
To assess the relationship between the results of the Sperm Quality Analyzer (United Medical Systems Inc., Santa Ana, CA), which measures motile sperm concentration by light scattering, conventional manual semen analysis characteristics, and computer-assisted sperm motility analyses.
Sperm Quality Analyzer measurements and manual and computer-assisted semen analyses were performed on 150 (50, 62, and 38) samples in three laboratories and the results were compared.
The study was performed in the Andrology Laboratory of Prince Henry's Institute of Medical Research, Monash Medical Centre, and Andrology Laboratory and Reproductive Biology Unit at the Royal Women's Hospital, Melbourne, Victoria, Australia.
Patients presented to the laboratories for routine fertility evaluation in the male and were selected at random to reflect the range of normal and abnormal samples seen in the laboratories.
None.
Sperm count, motility (percent motility, motility index, velocity, and amplitude of lateral head displacement [ALH]), morphology, and normal acrosomes were evaluated by manual and computer-assisted semen analysis and sperm quality analyzer motility index.
Spearman nonparametric univariate analysis showed strong correlations between sperm motility index and manual sperm concentration, motility, abnormal morphology, and normal acrosomes by Pisum sativum agglutinin; and computer-assisted sperm motility analysis sperm concentration, motile concentration, and percent static. Curvilinear velocity, straight-line velocity (VSL), and linearity also were related significantly to sperm motility index values. By multiple regression analysis, the significant covariates of the sperm motility index were motile sperm concentration, abnormal morphology, ALH, and straight-line velocity and these accounted for 85.5% of the variance of the sperm motility index.
The Sperm Quality Analyzer is easy to use. The good correlation between the sperm motility index, motile sperm concentration, and, in addition, a number of other semen parameters supports the use of the Sperm Quality Analyzer for screening patients and in situations that warrant a rapid verification of semen quality, such as in the IVF or artificial insemination clinic. Further investigation of the Sperm Quality Analyzer in the management of male infertility is warranted.
评估精子质量分析仪（联合医疗系统公司，加利福尼亚州圣安娜）通过光散射测量活动精子浓度的结果、传统手工精液分析特征以及计算机辅助精子活力分析之间的关系。
在三个实验室对150份（50份、62份和38份）样本进行精子质量分析仪测量以及手工和计算机辅助精液分析，并比较结果。
该研究在澳大利亚维多利亚州墨尔本莫纳什医疗中心亨利王子医学研究所男科实验室、皇家妇女医院男科实验室和生殖生物学科进行。
患者到实验室进行男性常规生育力评估，随机选取以反映实验室中正常和异常样本的范围。
无。
通过手工和计算机辅助精液分析以及精子质量分析仪活力指数评估精子计数、活力（活力百分比、活力指数、速度和头部侧向位移幅度[ALH]）、形态和正常顶体。
Spearman非参数单变量分析显示，精子活力指数与手工精子浓度、活力、异常形态以及豌豆凝集素检测的正常顶体之间存在强相关性；与计算机辅助精子活力分析的精子浓度、活动浓度和静止百分比也存在强相关性。曲线速度、直线速度（VSL）和线性度也与精子活力指数值显著相关。通过多元回归分析，精子活力指数的显著协变量为活动精子浓度、异常形态、ALH和直线速度，这些因素占精子活力指数方差的85.5%。
精子质量分析仪易于使用。精子活力指数、活动精子浓度以及许多其他精液参数之间的良好相关性支持在筛查患者以及在需要快速验证精液质量的情况下（如在体外受精或人工授精诊所）使用精子质量分析仪。有必要对精子质量分析仪在男性不育管理中的应用进行进一步研究。
