Instituto de Biología y Medicina Experimental (IBYME), National Research Council of Argentina (CONICET)-Fundación IBYME (FIBYME), Buenos Aires, Argentina.
Laboratorio de Andrología y Reproducción (LAR), Córdoba, Argentina.
Fertil Steril. 2018 Jul 1;110(1):68-75.e4. doi: 10.1016/j.fertnstert.2018.03.016.
To assess the impact of aging on routine semen and computer-assisted sperm analysis (CASA) motility parameters according to the current World Health Organization guidelines; and to evaluate the effect of obesity and lifestyle (alcohol consumption, cigarette smoking) in older men's semen.
Blind cross-sectional study.
Research laboratory and andrology and reproduction laboratory.
PATIENT(S): A population of 11,706 men.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Semen analysis: routine (semen volume, sperm concentration and count, motility, vitality, morphology, hypo-osmotic swelling test, round and peroxidase-positive cell concentration) and CASA (straight-line velocity, curvilinear velocity, average path velocity, linearity, straightness, beat cross frequency, wobble, amplitude of lateral head displacement, and mean angular displacement) parameters; and body mass index.
RESULT(S): A negative correlation was found between age and routine semen parameters: volume, sperm count, motility, vitality, total motile spermatozoa and normal-motile spermatozoa, round cell concentration, and hypo-osmotic swelling test values. Several CASA variables (straight-line velocity, curvilinear velocity, average path velocity, beat cross frequency, amplitude of lateral head displacement, and mean angular displacement) were also negatively affected. Using 40 years as a cut-off value, significant differences in most parameters correlated to age. In a selected subpopulation of men unexposed to known fertility-compromising factors, the same evaluations were performed, finding some parameters still decreased. Although obesity exerted a significant deleterious effect on older patients' semen quality, alcohol consumption and cigarette smoking mildly affected it.
CONCLUSION(S): Male aging, with the contribution of unhealthy conditions, are paramount effectors of sperm quality deterioration.
根据世界卫生组织（WHO）的现行指南，评估年龄对常规精液和计算机辅助精子分析（CASA）活力参数的影响；并评估肥胖和生活方式（饮酒、吸烟）对老年男性精液的影响。
盲法横断面研究。
研究实验室和男科及生殖实验室。
患者（或人群）：11706 名男性。
无。
精液分析：常规参数（精液量、精子浓度和计数、活力、活力、形态、低渗肿胀试验、直线速度、曲线速度、平均路径速度、直线性、直线性、鞭打频率、摆动、头部侧向位移振幅和平均角位移）和 CASA（直线速度、曲线速度、平均路径速度、直线性、直线性、鞭打频率、摆动、头部侧向位移振幅和平均角位移）参数；以及体重指数。
年龄与常规精液参数呈负相关：体积、精子计数、活力、活力、总活动精子和正常活动精子、圆形细胞浓度和低渗肿胀试验值。几个 CASA 变量（直线速度、曲线速度、平均路径速度、鞭打频率、头部侧向位移振幅和平均角位移）也受到负面影响。以 40 岁为截止值，与年龄相关的大多数参数差异显著。在未接触已知生育能力受损因素的男性亚群中进行了相同的评估，发现一些参数仍在下降。尽管肥胖对老年患者的精液质量有显著的不良影响，但饮酒和吸烟对其影响较小。
男性衰老，加上不健康状况的影响，是精子质量恶化的主要因素。
