Intracytoplasmic sperm injection, fertilization, and embryo transfer after retrieval of spermatozoa by testicular biopsy from an azoospermic male with testicular tubular atrophy.

OBJECTIVE

To achieve fertilization and cleavage by spermatozoa retrieved by testicular biopsy from a male with testicular tubular atrophy.

DESIGN

Clinical trial.

SETTING

Private reproductive institute.

PATIENT

Azoospermic male with demonstrated testicular tubular atrophy and almost complete spermatogenic arrest.

INTERVENTION

Open biopsy retrieval of testicular tissue and sperm followed by intracytoplasmic sperm injection.

MAIN OUTCOME MEASURES

Fertilization and cleavage.

RESULTS

One four- to six-cell embryo was formed after intracytoplasmic sperm injection of five eggs with extruded polar bodies by retrieved sperm.

CONCLUSION

Intracytoplasmic sperm injection after testicular sperm aspiration may be attempted in cases with severely decreased spermatogenesis and result in fertilization, cleavage, and embryo transfer.