Romero J, Remohí J, Mínguez Y, Rubio C, Pellicer A, Gil-Salom M
Instituto Valenciano de Infertilidad and Facultad de Medicina, University of Valencia, Spain.
Fertil Steril. 1996 Apr;65(4):877-9. doi: 10.1016/s0015-0282(16)58232-6.
To assess the possibility of cryopreserving testicular tissue extracted sperm for intracytoplasmic sperm injection (ICSI).
A report of two cases. Our study was approved by the Ethical Committee at the Instituto Valenciano de Infertilidad.
In vitro fertilization program at the Instituto Valenciano de Infertilidad.
Two azoospermic patients with severe spermatogenic failure but with focal spermatogenesis on testicular biopsies. In both cases, a first ICSI attempt with fresh testicular biopsy extracted sperm was unsuccessful.
Cryopreservation of testicular spermatozoa in 100-micro L "pills." Intracytoplasmic sperm injection with thawed testicular spermatozoa.
Fertilization rate, cleavage rate, embryo quality, clinical pregnancy.
Fertilization rates were 36 percent and 100 percent after ICSI with fresh testicular spermatozoa, and 63 percent and 57 percent after ICSI with cryopreserved testicular sperm. In both cases, cleavage rates and embryo quality were similar when using fresh and cryopreserved testicular spermatozoa. No clinical pregnancies were achieved.
High fertilization rates can be obtained after ICSI with frozen-thawed testicular tissue extracted spermatozoa. Cryopreservation of testicular sperm may avoid repetition of testicular biopsies in azoospermic patients in whom the only source of spermatozoa is the testicle.
评估冷冻保存从睾丸组织中提取的精子用于卵胞浆内单精子注射（ICSI）的可能性。
两例病例报告。我们的研究得到了瓦伦西亚不孕症研究所伦理委员会的批准。
瓦伦西亚不孕症研究所的体外受精项目。
两名无精子症患者，严重生精功能衰竭，但睾丸活检显示有局灶性生精。两例患者首次尝试使用新鲜睾丸活检提取的精子进行ICSI均未成功。
将睾丸精子以100微升“药丸”形式冷冻保存。使用解冻后的睾丸精子进行卵胞浆内单精子注射。
受精率、卵裂率、胚胎质量、临床妊娠情况。
使用新鲜睾丸精子进行ICSI后的受精率分别为36%和100%，使用冷冻保存的睾丸精子进行ICSI后的受精率分别为63%和57%。在这两例中，使用新鲜和冷冻保存的睾丸精子时，卵裂率和胚胎质量相似。均未实现临床妊娠。
使用冷冻解冻后的睾丸组织提取的精子进行ICSI后可获得较高的受精率。对于无精子症且精子唯一来源是睾丸的患者，冷冻保存睾丸精子可避免重复进行睾丸活检。
