去纤苷片段的纤溶活性研究。

Investigation of the fibrinolytic activity of defibrotide fractions.

Hizal N, Bilsel S, Emerk K

Department of Biochemistry, Faculty of Medicine, Marmara University, Istanbul, Turkey.

Gen Pharmacol. 1994 Dec;25(8):1617-20. doi: 10.1016/0306-3623(94)90363-8.

Abstract

Defibrotide is a drug which is a mixture of DNA fragments of various lengths. To characterize the active component which is responsible for the fibrinolytic effect, we fractionated defibrotide by column chromatography and investigated direct and endothelium dependent fibrinolytic activity of the major fractions by euglobulin lysis time (ELT) measurements. 2. Defibrotide and its fractions added to human plasma samples did not change ELT. However addition of conditioned media of human umbilical vein endothelial cell cultures which were incubated with defibrotide or its high molecular weight fractions shortened the ELT significantly (P < 0.001), whereas small molecular weight fractions did not effect the ELT. 3. Our results show that high molecular weight defibrotide fractions have fibrinolytic activity and the effect is endothelium dependent.

摘要

去纤苷是一种由各种长度的DNA片段组成的混合物。为了表征负责纤溶作用的活性成分，我们通过柱色谱法对去纤苷进行了分级分离，并通过优球蛋白溶解时间（ELT）测量研究了主要级分的直接和内皮依赖性纤溶活性。2. 添加到人体血浆样本中的去纤苷及其级分并未改变ELT。然而，添加与去纤苷或其高分子量级分一起孵育的人脐静脉内皮细胞培养物的条件培养基可显著缩短ELT（P < 0.001），而小分子级分则不影响ELT。3. 我们的结果表明，高分子量去纤苷级分具有纤溶活性，且该作用是内皮依赖性的。