Lysenko E S, Ogarkova O A, Elanskaia I V, Tarasov V A, Shestakov S V
Genetika. 1995 Feb;31(2):162-9.
A new open reading frame ORF242, coding for a 26.47-kDa polypeptide, was found in a DNA fragment of the cyanobacterium Synechocystis 6803, transforming a photosynthetic mutant to photoautotrophy and having homology with plant chloroplast DNA. In the 5' flanking region of ORF242, consensus sequences characteristic of a functioning gene were found. One copy of ORF242 is present in the Synechocystis 6803 genome. Insertion inactivation of ORF242 does not lead to a decrease in photosynthetic activity in cells of cyanobacteria but may influence the ratio between active complexes of photosystems I and II.
在集胞藻6803的一个DNA片段中发现了一个新的开放阅读框ORF242,它编码一种26.47 kDa的多肽,能将光合突变体转化为光合自养型,并且与植物叶绿体DNA具有同源性。在ORF242的5'侧翼区域,发现了功能基因特有的共有序列。集胞藻6803基因组中存在一个ORF242拷贝。ORF242的插入失活不会导致蓝藻细胞光合活性降低,但可能会影响光系统I和II活性复合物之间的比例。