Shark K B, Lee N M
Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA.
Gene. 1995 Apr 3;155(2):213-7. doi: 10.1016/0378-1119(94)00830-l.
Oligodeoxyribonucleotide (oligo) primers derived from rat opioid-binding cell adhesion molecule (OBCAM)-encoding cDNA sequence were used to amplify a 403-bp fragment from a human brain cDNA library using the polymerase chain reaction (PCR). The fragment was cloned, sequenced and used as a hybridization probe to screen the library. lambda plaque clones were isolated which contained a 1.5-kb cDNA fragment, including a complete open reading frame (ORF) of 1038 bp. Sequence analysis of the ORF revealed 93% identity to the rat OBCAM cDNA at the nucleotide level, and the deduced amino-acid sequences shared 98% identity. Percentages of identity between human and bovine OBCAM ORFs were within 2% of these values. OBCAM was mapped to human chromosome 11 by hybridizing the probe with a somatic cell hybrid panel.
从大鼠阿片样物质结合细胞粘附分子（OBCAM）编码cDNA序列衍生的寡脱氧核糖核苷酸（oligo）引物，用于通过聚合酶链反应（PCR）从人脑cDNA文库中扩增出一个403bp的片段。该片段被克隆、测序，并用作杂交探针筛选文库。分离出λ噬菌斑克隆，其包含一个1.5kb的cDNA片段，其中包括一个1038bp的完整开放阅读框（ORF）。对该ORF的序列分析显示，在核苷酸水平上与大鼠OBCAM cDNA的同源性为93%，推导的氨基酸序列同源性为98%。人和牛OBCAM ORF之间的同源性百分比在这些值的2%以内。通过将探针与体细胞杂交板杂交，将OBCAM定位于人类11号染色体。
