Guzder S N, Bailly V, Sung P, Prakash L, Prakash S
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555-1061, USA.
J Biol Chem. 1995 Apr 14;270(15):8385-8. doi: 10.1074/jbc.270.15.8385.
In Saccharomyces cerevisiae, the multisubunit RNA polymerase II general transcription factor TFIIH is indispensable for transcription initiation and some of its subunits are known to be required for nucleotide excision repair (NER) of DNA damaged by ultraviolet light. RAD3, a subunit of TFIIH, binds UV-damaged DNA in an ATP-dependent manner. It has, however, remained unclear how TFIIH is assembled with the other damage recognition component RAD14. Here, we demonstrate a higher order complex consisting of TFIIH, RAD14, and another NER protein RAD23, and complex formation between TFIIH and RAD14 is facilitated by the RAD23 protein.
在酿酒酵母中，多亚基RNA聚合酶II通用转录因子TFIIH对于转录起始是不可或缺的，并且已知其一些亚基是紫外线损伤DNA的核苷酸切除修复（NER）所必需的。TFIIH的一个亚基RAD3以ATP依赖的方式结合紫外线损伤的DNA。然而，尚不清楚TFIIH是如何与另一个损伤识别组分RAD14组装在一起的。在这里，我们证明了由TFIIH、RAD14和另一个NER蛋白RAD23组成的高阶复合物，并且RAD23蛋白促进了TFIIH与RAD14之间的复合物形成。
