Nataf P, Guettier C, Martinelli L, Regan M, Pavie A, Cabrol C, Gandjbakhch I
Department of Cardiovascular Surgery, La Pitié Hospital, Paris, France.
J Cardiovasc Surg (Torino). 1995 Feb;36(1):79-85.
The search of small-caliber vascular prosthesis for myocardial revascularization and arterial reconstruction has led to an investigation of cryopreserved arterial allografts in a sheep model. Carotid arteries with an internal diameter of 4 mm and length of 10 cm were harvested from donor sheep and soaked immediately in a cold saline solution. Nine arteries were cryopreserved in a nutrient media containing 10% DMSO as cryoprotectant and then were stored in a liquid nitrogen vapor at -150 degrees C to -170 degrees C. In recipient sheep from different species, arterial substitution of a 10 cm segment of carotid artery was realised by implantation of fresh (n = 9) or cryopreserved (n = 9) carotid artery allografts. After 3 months, the allografts were harvested. In both groups: 7/9 were patent at the time of explantation. Components of arterial allograft rejection were observed in most fresh and cryopreserved arteries: intimal thickening with cell proliferation was seen in fresh (3/7) and cryopreserved (4/8) arteries; Loss of smooth muscle medial cells was constant; Adventitia was involved by a marked inflammatory reaction. A non cellular fibrous intimal thickening and presence of large medial calcifications were essentially observed in cryopreserved arteries. In conclusion, these results show that at three months post implantation, there is little difference in the outcome of fresh and cryopreserved arterial allografts. Cryopreservation does not reduce the apparent antigenicity of small diameter arterial allografts.
为寻找用于心肌血运重建和动脉重建的小口径血管移植物，人们在绵羊模型中对冷冻保存的同种异体动脉进行了研究。从供体绵羊身上采集内径为4毫米、长度为10厘米的颈动脉，立即浸泡在冷盐溶液中。九条动脉在含有10%二甲基亚砜作为冷冻保护剂的营养培养基中进行冷冻保存，然后储存在-150℃至-170℃的液氮蒸汽中。在不同物种的受体绵羊中，通过植入新鲜（n = 9）或冷冻保存（n = 9）的颈动脉同种异体移植物实现了10厘米长颈动脉段的动脉置换。3个月后，取出移植物。在两组中：9条移植物中有7条在取出时通畅。在大多数新鲜和冷冻保存的动脉中都观察到了同种异体动脉排斥反应的成分：新鲜（3/7）和冷冻保存（4/8）的动脉中可见内膜增厚伴细胞增殖；平滑肌中层细胞持续丢失；外膜有明显的炎症反应。在冷冻保存的动脉中主要观察到无细胞纤维内膜增厚和大量中层钙化。总之，这些结果表明，植入后三个月，新鲜和冷冻保存的同种异体动脉移植物的结果几乎没有差异。冷冻保存不会降低小口径同种异体动脉移植物的明显抗原性。
