Vijtiuk N, Curić S, Lacković G, Udovicić I, Vrbanac I, Valpotić I
Department of Biology, Veterinary Faculty, University of Zagreb, Croatia.
J Comp Pathol. 1995 Jan;112(1):1-10. doi: 10.1016/s0021-9975(05)80085-4.
Four porcine strains of Escherichia coli were examined for their effects on the small intestine of 4-week-old weaned pigs infected orogastrically. The strains used experimentally were: strain 1467 (adhesin negative, non-toxigenic); strains 2407 and 1466 (adhesin positive, non-toxigenic), derived by genetical engineering from strain 1467 and containing a wild type plasmid and a recombinant plasmid, respectively, encoding the F4 antigen (adhesin); and strain M1823 (adhesin positive, toxigenic). In addition, 2-week-old pigs that died from natural colibacillosis associated with two strains ("Ihan 1 and 2"; adhesin positive, toxigenic) were examined. Strain M1823 and the Ihan strains produced moderate and marked lesions, respectively. Strain 1467 did not cause mucosal damage or an inflammatory response. Strains 1466 and 2407 caused a mild to moderate leucocyte (mononuclear and polymorphonuclear) infiltration in the jejunal (but not ileal) lamina propria. However, unlike strain 1466, strain 2407 did not cause damage to the small intestinal mucosa and should be further studied as a potential oral vaccine strain for post-weaning E. coli diarrhoea.
对4株猪源大肠杆菌进行了研究，观察它们对经口胃途径感染的4周龄断奶仔猪小肠的影响。实验所用菌株如下：1467株（无黏附素，无毒素）；2407株和1466株（有黏附素，无毒素），分别由基因工程从1467株衍生而来，分别含有一个野生型质粒和一个重组质粒，编码F4抗原（黏附素）；以及M1823株（有黏附素，产毒素）。此外，还检查了死于与两株菌（“伊汉1和2”；有黏附素，产毒素）相关的自然大肠杆菌病的2周龄仔猪。M1823株和伊汉菌株分别产生了中度和明显的病变。1467株未引起黏膜损伤或炎症反应。1466株和2407株在空肠（而非回肠）固有层引起轻度至中度白细胞（单核和多形核）浸润。然而，与1466株不同，2407株未对小肠黏膜造成损伤，作为断奶后大肠杆菌腹泻的潜在口服疫苗株应进一步研究。
