Wastling J M, Harkins D, Maley S, Innes E, Panton W, Thomson K, Buxton D
Moredun Research Institute, Edinburgh, UK.
J Comp Pathol. 1995 Jan;112(1):53-62. doi: 10.1016/s0021-9975(05)80089-1.
Vaccination of sheep with live tachyzoites of Toxoplasma gondii, strain S48, affords protection against subsequent challenge with the parasite, but the mechanisms of immunity have not been fully determined. To understand better the nature of the antibody response the kinetics of both local and systemic antibody production were monitored in vaccinated sheep by means of an enzyme-linked immunosorbent assay and Western blotting. Local specific IgG production was analysed in efferent lymph obtained from the cannulated pre-femoral lymph node draining the site of infection. Antibody in efferent lymph plasma and peripheral blood serum from animals vaccinated with S48 tachyzoites was monitored and compared with IgG production in vaccinated sheep given a secondary tachyzoite challenge. Secondary challenge resulted in a clear immunological memory response, antibody being detected in the lymph 3 to 4 days after infection as compared with 7 to 8 days after a primary infection. IgG production was dominated by antibody recognizing a protein with an apparent molecular weight of 30 kDa, but other antigens (32, 24 and 11 kDa) were also readily detected.
用刚地弓形虫S48株的活速殖子对绵羊进行疫苗接种，可使其免受该寄生虫随后的攻击，但免疫机制尚未完全明确。为了更好地了解抗体反应的本质，通过酶联免疫吸附测定和蛋白质印迹法监测了接种疫苗的绵羊局部和全身抗体产生的动力学。在从引流感染部位的插管股前淋巴结获得的输出淋巴中分析局部特异性IgG的产生。监测接种S48速殖子的动物输出淋巴血浆和外周血血清中的抗体，并与再次接受速殖子攻击的接种绵羊的IgG产生情况进行比较。再次攻击导致明显的免疫记忆反应，感染后3至4天在淋巴中检测到抗体，而初次感染后为7至8天。IgG的产生主要由识别表观分子量为30 kDa的蛋白质的抗体主导，但其他抗原（32、24和11 kDa）也很容易被检测到。
