Innes E A, Panton W R, Thomson K M, Maley S, Buxton D
Moredun Research Institute, Edinburgh, UK.
J Comp Pathol. 1995 Jul;113(1):89-94. doi: 10.1016/s0021-9975(05)80074-x.
The kinetics of interferon gamma (IFN gamma) production in vivo was examined in sheep during a primary and secondary infection with the sheep vaccine strain (S48) of Toxoplasma gondii. Efferent lymph plasma from a node draining the site of inoculation was tested for anti-viral activity which could be neutralized with monoclonal antibodies against IFN gamma. Within 2 to 5 days of primary infection IFN gamma was detected in each of five sheep and persisted for 6 to 9 days. Accelerated production of IFN gamma occurred after secondary infection, the cytokine being detected in the first 24 h, and persisting in lymph for a further 4-5 days. From day 6 onwards after primary infection, efferent lymph cells produced IFN gamma when stimulated in vitro with a crude T. gondii antigen. These results show that IFN gamma is induced in sheep after infection with the S48 strain of T. gondii.
在绵羊初次和再次感染刚地弓形虫绵羊疫苗株（S48）期间，对其体内γ干扰素（IFNγ）的产生动力学进行了研究。对接种部位引流淋巴结的输出淋巴血浆进行抗病毒活性检测，该活性可被抗IFNγ单克隆抗体中和。初次感染后2至5天内，在五只绵羊中均检测到IFNγ，并持续6至9天。再次感染后IFNγ产生加速，在最初24小时内即可检测到该细胞因子，并在淋巴中持续存在4至5天。初次感染后第6天起，输出淋巴细胞在体外用粗制刚地弓形虫抗原刺激时产生IFNγ。这些结果表明，绵羊感染刚地弓形虫S48株后可诱导产生IFNγ。
