Ebersole J L, Cappelli D, Sandoval M N, Steffen M J
Department of Periodontics, University of Texas Health Science Center, San Antonio 78284, USA.
J Dent Res. 1995 Feb;74(2):658-66. doi: 10.1177/00220345950740020601.
We hypothesized that serum antibody with selected antigen specificities would relate to infection and disease in the patients and, thus, describe the characteristics of potential protective antibody. This study used serum samples from 24 periodontitis patients with subgingival infection and elevated serum IgG antibody to A. actinomycetemcomitans to define the antigenic specificities of IgG, IgM, IgA, and IgG1-4 antibody to A. actinomycetemcomitans strain Y4 outer membrane antigens (OMA). Uniform IgG antibody (> 70% of the patients) was noted to antigens with M(r) of 65, 38, 29, and 17 kDa. Both IgA and IgM specificities reflected those shown for IgG in each patient. IgG1 and IgG2 antibody reacted with several OMA bands in each patient, while IgG3 antibodies were directed to numerous OMA bands in many patients and represented the most broad-based response. The IgG4 response patterns were limited to a few OMA bands. We noted a prominent occurrence of IgG reactions with OMA bands that were characteristic for individual patients. The frequency of responses to OMA of higher M(r) (i.e., > 80 kDa) and to the 34-, 31-, and 24-kDa antigens was positively related to the total IgG antibody levels. Antibody reactive with OMA bands at 65-, 38-, 29-, 17-, 15-, and 11-kDa antigens was detected in patients with few to many teeth infected with A. actinomycetemcomitans. Furthermore, patients with a high percentage of teeth with > or = 6 mm pockets had a decreased frequency of responses to the high-M(r) antigens (i.e. > 90 kDa) as well as to the 58-kDa antigen.(ABSTRACT TRUNCATED AT 250 WORDS)
我们推测，具有特定抗原特异性的血清抗体与患者的感染及疾病相关，进而可描述潜在保护性抗体的特征。本研究使用了24例患有龈下感染且血清抗伴放线放线杆菌IgG抗体升高的牙周炎患者的血清样本，以确定IgG、IgM、IgA及IgG1 - 4抗体针对伴放线放线杆菌Y4菌株外膜抗原（OMA）的抗原特异性。发现针对分子量为65、38、29和17 kDa抗原的IgG抗体一致（超过70%的患者）。每位患者的IgA和IgM特异性均反映出与IgG相同的情况。每位患者的IgG1和IgG2抗体与多条OMA条带发生反应，而IgG3抗体在许多患者中针对众多OMA条带，呈现出最广泛的反应。IgG4的反应模式局限于少数OMA条带。我们注意到，IgG与对个体患者具有特征性的OMA条带反应显著。对较高分子量（即> 80 kDa）的OMA以及对34、31和24 kDa抗原的反应频率与总IgG抗体水平呈正相关。在感染伴放线放线杆菌牙齿数量不等的患者中，均检测到与65、38、29、17、15和11 kDa抗原的OMA条带发生反应的抗体。此外，牙周袋≥6 mm牙齿比例高的患者，对高分子量抗原（即> 90 kDa）以及对58 kDa抗原的反应频率降低。（摘要截于250字）
