Desmettre T, Mordon S, Devoiselle J M, Constantinides G, Brunetaud J M
Service d'Ophtalmologie, CHRU, Lille.
J Fr Ophtalmol. 1994;17(12):774-80.
The aim of this in-vivo/ex vivo study was to assess the possible use of temperature sensitive liposomes in an established model such as the liver as a new approach to monitor the temperature induced by a laser.
Temperature sensitive liposomes (DSPC: DiStearoylPhophatidyl-Choline: critical temperature: 55 degrees C) loaded with carboxy-fluoresceine were prepared by the Bancham procedure. These liposomes (1 ml solution) were injected to adult male wistar rats. Two hours later, the liver was exposed and irradiated with a 100 W Nd: YAG laser (single pulse mode, pulses ranging from 100 to 260 ms, spot diameter: 4 mm). Simultaneously, the surface temperature was recorded with a infrared thermographic camera (Aga). The fluorescence emission was then measured with a fluorescent imaging system (Hamamatsu).
The dye was released in response to laser energy. The amount of the drug release increased linearly with increasing temperature in the range 42 to 60 degrees C. The estimated latency of release seems more related to the heating pattern (i.e. to laser parameters) than to the liposomes.
This preliminary study assesses the possible use of laser-induced release of liposome-encapsulated dye to monitor tissue temperature and then thermal damage.
本体内/体外研究的目的是评估温度敏感脂质体在诸如肝脏这样的既定模型中作为监测激光诱导温度的新方法的可能用途。
采用班汉姆法制备负载羧基荧光素的温度敏感脂质体（DSPC：二硬脂酰磷脂酰胆碱：临界温度：55摄氏度）。将这些脂质体（1毫升溶液）注射到成年雄性Wistar大鼠体内。两小时后，暴露肝脏并用100瓦钕：钇铝石榴石激光照射（单脉冲模式，脉冲范围为100至260毫秒，光斑直径：4毫米）。同时，用红外热成像相机（阿加）记录表面温度。然后用荧光成像系统（滨松）测量荧光发射。
染料响应激光能量而释放。在42至60摄氏度范围内，药物释放量随温度升高呈线性增加。估计的释放潜伏期似乎与加热模式（即激光参数）的关系比与脂质体的关系更大。
这项初步研究评估了激光诱导释放脂质体包裹染料以监测组织温度进而监测热损伤的可能用途。
