De Inocencio J, Choi E, Glass D N, Hirsch R
Division of Rheumatology, Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
J Immunol. 1995 May 1;154(9):4836-41.
The generation of TCR diversity occurs primarily through rearrangement of germline DNA. Genetic polymorphism of the TCR chains appears to be a rarer mechanism for generating repertoire differences between races. Flow cytometric analysis of the TCR V beta repertoire in a population of healthy African Americans (n = 30) and Caucasians (n = 30) revealed a significant difference in the frequency of cells bearing V beta 3.1, but not V beta 2, V beta 5.1, V beta 5.2-5.3, V beta 6.7, V beta 8.1-8.2, V beta 12.1, V beta 13.3, or V beta 19. African Americans had a significantly lower frequency of V beta 3.1+ cells, in both the CD4+ (2.55 +/- 0.36% vs 4.85 +/- 0.43%, p = 0.0001) and the CD8+ (3.03 +/- 0.54% vs 5.32 +/- 0.57%, p = 0.004) population than did Caucasians, and this difference was independent of the age of the individuals. Analysis of genomic DNA revealed that the observed difference in frequency of V beta 3.1+ cells correlated with a recently described polymorphism of the recombination signal sequence of the TCRBV3S1 gene. Allele 1, associated with a lower frequency of V beta 3.1+ cells, was more commonly present in African Americans (0.68 vs 0.43), whereas allele 2, associated with a higher frequency of V beta 3.1+ cells, was more commonly present in Caucasians (0.31 vs 0.56). This study demonstrates the potential for TCR repertoire differences, based on genetic polymorphism, between African Americans and Caucasians.
TCR多样性的产生主要通过种系DNA的重排。TCR链的基因多态性似乎是一种较为罕见的在不同种族间产生库差异的机制。对30名健康非裔美国人和30名高加索人的群体进行TCR Vβ库的流式细胞术分析显示，携带Vβ3.1的细胞频率存在显著差异，但Vβ2、Vβ5.1、Vβ5.2 - 5.3、Vβ6.7、Vβ8.1 - 8.2、Vβ12.1、Vβ13.3或Vβ19不存在差异。非裔美国人中Vβ3.1 +细胞的频率在CD4 +群体（2.55±0.36%对4.85±0.43%，p = 0.0001）和CD8 +群体（3.03±0.54%对5.32±0.57%，p = 0.004）中均显著低于高加索人，且这种差异与个体年龄无关。对基因组DNA的分析表明，观察到的Vβ3.1 +细胞频率差异与最近描述的TCRBV3S1基因重组信号序列的多态性相关。与Vβ3.1 +细胞频率较低相关的等位基因1在非裔美国人中更常见（0.68对0.43），而与Vβ3.1 +细胞频率较高相关的等位基因2在高加索人中更常见（0.31对0.56）。这项研究证明了基于基因多态性，非裔美国人和高加索人之间TCR库存在差异的可能性。
