Kubota Y, Nakamura A, Ito M, Shiojiri N
Department of Biology, Faculty of Science, Shizuoka University, Japan.
J Exp Zool. 1995 Apr 15;271(6):441-51. doi: 10.1002/jez.1402710605.
Genotypes of early embryos obtained from mating Bh/+ quail were determined by chorio-allantoic membrane grafting of their thigh skin, and the development of the Bh homozygous embryos was examined histologically. The Bh homozygous embryos died at an early stage of development (after 7 to 10 days of incubation) with a very high incidence of whole-body hemorrhage (approximately 100%), but malformation of neural tubes was not always accompanied by Bh homozygosity. Degeneration of the liver was more frequently observed in homozygous embryos than in wild-type and heterozygous embryos. The results suggest that the Bh gene causes whole-body hemorrhage and subsequent death of homozygous embryos. In addition, our data indicate that Bh homozygotes would develop brown feathers on their skin if they were able to survive.
通过对Bh/+鹌鹑交配所获早期胚胎的大腿皮肤进行绒毛尿囊膜移植，确定其基因型，并对Bh纯合胚胎的发育进行组织学检查。Bh纯合胚胎在发育早期（孵化7至10天后）死亡，全身出血发生率极高（约100%），但神经管畸形并不总是与Bh纯合性相伴。与野生型和杂合子胚胎相比，在纯合胚胎中更常观察到肝脏退化。结果表明，Bh基因导致纯合胚胎全身出血并随后死亡。此外，我们的数据表明，如果Bh纯合子能够存活，其皮肤上会长出棕色羽毛。
