神经生长因子（NGF）和脑源性神经营养因子（BDNF）对胚胎基底前脑神经元电压门控钙电流的不同影响。
Differential effects of NGF and BDNF on voltage-gated calcium currents in embryonic basal forebrain neurons.
作者信息
Levine E S, Dreyfus C F, Black I B, Plummer M R
机构信息
Department of Neuroscience and Cell Biology, UMDNJ/Robert Wood Johnson Medical School, Piscataway 08854, USA.
出版信息
J Neurosci. 1995 Apr;15(4):3084-91. doi: 10.1523/JNEUROSCI.15-04-03084.1995.
A number of studies have begun to describe the effects of nerve growth factor (NGF) and the closely related brain-derived neurotrophic factor (BDNF) on the function of basal forebrain neurons. Little is known, however, about the effects of neurotrophins on membrane calcium conductances, which may play a role in growth factor signal transduction as well as regulation of neuronal excitability. Using the whole-cell patch-clamp technique, we investigated the effects of both NGF and BDNF on voltage-gated Ca(2+)-channel currents in cultured embryonic basal forebrain neurons. Exposure for 4-6 d to NGF significantly increased both the L-type and N-type components of the whole-cell current. Conversely, similar exposure to BDNF had no effect on Ca(2+)-channel currents. Consequently, one of the important effects of NGF may be to enhance calcium entry via voltage-dependent channels.
多项研究已开始描述神经生长因子（NGF）以及密切相关的脑源性神经营养因子（BDNF）对基底前脑神经元功能的影响。然而，对于神经营养因子对膜钙电导的影响却知之甚少，而膜钙电导可能在生长因子信号转导以及神经元兴奋性调节中发挥作用。我们运用全细胞膜片钳技术，研究了NGF和BDNF对培养的胚胎基底前脑神经元电压门控Ca(2+)通道电流的影响。将细胞暴露于NGF 4 - 6天可显著增加全细胞电流的L型和N型成分。相反，类似地暴露于BDNF对Ca(2+)通道电流没有影响。因此，NGF的重要作用之一可能是增强通过电压依赖性通道的钙内流。
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