Jain A, Madsen D C, Auld P A, Frayer W W, Schwartz M K, Meister A, Mårtensson J
Perinatology Center/Department of Pediatrics, Cornell University Medical College, New York, NY 10021, USA.
J Nutr. 1995 Apr;125(4):851-6. doi: 10.1093/jn/125.4.851.
The efficiency of L-2-oxothiazolidine-4-carboxylate, a cysteine precursor, in stimulating glutathione synthesis and growth was evaluated in growing rats. Animals were fed a sulfur amino acid-deficient diet (0.25% L-methionine and no cysteine) supplemented with L-2-oxothiazolidine-4-carboxylate (0.35%) for 3 wk and compared with age-matched animals receiving the sulfur amino acid-deficient diet alone. Rats fed the sulfur amino acid-deficient diet had lower glutathione concentrations in bronchoalveolar lining fluid, lung, lymphocytes, and liver than rats fed a sulfur amino acid-deficient diet supplemented with L-2-oxothiazolidine-4-carboxylate. Rats fed the supplemented diet had normal tissue and bronchoalveolar lining fluid glutathione levels. Central venous plasma glutathione concentrations, mostly reflecting liver excretion, were less affected by L-2-oxothiazolidine-4-carboxylate supplementation. Rats fed L-2-oxothiazolidine-4-carboxylate supplementation had normal weight gain compared with a much lower weight gain in animals fed the sulfur amino acid-deficient diet alone. Thus, L-2-oxothiazolidine-4-carboxylate increased tissue glutathione concentrations and stimulated growth in rats. The lung glutathione status of the rats was reflected by glutathione concentrations in lymphocytes and the bronchoalveolar lining fluid, but not by the central venous plasma glutathione concentrations.
在生长中的大鼠身上评估了半胱氨酸前体L-2-氧代噻唑烷-4-羧酸刺激谷胱甘肽合成及生长的效率。给动物喂食一种缺乏硫氨基酸的饮食（0.25% L-甲硫氨酸且无半胱氨酸），并补充L-2-氧代噻唑烷-4-羧酸（0.35%），持续3周，然后与仅接受缺乏硫氨基酸饮食的年龄匹配动物进行比较。与喂食补充了L-2-氧代噻唑烷-4-羧酸的缺乏硫氨基酸饮食的大鼠相比，喂食缺乏硫氨基酸饮食的大鼠，其支气管肺泡衬液、肺、淋巴细胞和肝脏中的谷胱甘肽浓度较低。喂食补充饮食的大鼠其组织和支气管肺泡衬液中的谷胱甘肽水平正常。主要反映肝脏排泄情况的中心静脉血浆谷胱甘肽浓度受L-2-氧代噻唑烷-4-羧酸补充的影响较小。与仅喂食缺乏硫氨基酸饮食的动物体重增加低得多相比，喂食补充了L-2-氧代噻唑烷-4-羧酸的大鼠体重增加正常。因此，L-2-氧代噻唑烷-4-羧酸提高了大鼠组织中的谷胱甘肽浓度并刺激了其生长。大鼠的肺谷胱甘肽状态可通过淋巴细胞和支气管肺泡衬液中的谷胱甘肽浓度反映，但不能通过中心静脉血浆谷胱甘肽浓度反映。
