Sobus M T, Homlund C E
Lipids. 1976 Apr;11(4):341-8. doi: 10.1007/BF02544064.
Several methods for the extraction of lipids from intact yeast cells have been compared. Extraction of intact cells with methanol followed by methanol: benzene (1:1, v/v) and benzene resulted in the recovery of equal or greater amounts of polar and nonpolar lipids than obtained by other methods. A preparative method involving preincubation of cells with aqueous KOH followed by the treatment of the cellular residue as described above yielded slightly more steryl esters than was extracted from broken cell preparations.
已对从完整酵母细胞中提取脂质的几种方法进行了比较。用甲醇提取完整细胞，然后用甲醇：苯（1:1，v/v）和苯提取，与其他方法相比，可回收等量或更多的极性和非极性脂质。一种制备方法是先将细胞与氢氧化钾水溶液预孵育，然后按上述方法处理细胞残渣，该方法产生的甾醇酯比从破碎细胞制剂中提取的略多。
