Cloning and expression of a bovine glutamate transporter.
作者信息
Inoue K, Sakaitani M, Shimada S, Tohyama M
机构信息
Department of Anatomy and Neuroscience, Osaka University, Medical School, Japan.
出版信息
Brain Res Mol Brain Res. 1995 Feb;28(2):343-8. doi: 10.1016/0169-328x(94)00244-9.
PMID:7723632
Abstract
We have isolated a 3845 base-pair cDNA (BNGLUAS) encoding a bovine glutamate transporter (bovine GLAST) by screening a bovine retina cDNA library with an oligonucleotide probe corresponding to conserved regions of known glutamate transporters. The cDNA sequence predicted a protein of 542 amino acids and displayed 96% and 97% amino acid identity with the rat GLAST/GluT-1 and human GLAST, respectively. Expression of the bovine GLAST in Xenopus oocytes revealed Na(+)-dependent [14C]L-glutamate uptake and electrogenic glutamate uptake.
摘要
Zotero 插件