Tanaka K
Laboratory for Neural Networks, Institute of Physical and Chemical Research, RIKEN, Saitama, Japan.
Neurosci Lett. 1993 Sep 3;159(1-2):183-6. doi: 10.1016/0304-3940(93)90829-a.
A complementary DNA clone encoding a transporter for glutamate has been isolated from mouse brain, and its functional properties have been examined in Xenopus oocytes. The transporter displays high affinity for glutamate (Km = 72 microM) and is dependent on external Na+. The cDNA sequence predicts a protein of 543 amino acids with 6 potential transmembrane domains. Northern blot analysis indicates the mRNA encoding the glutamate transporter is expressed both in the brain and in peripheral tissues.
一个编码谷氨酸转运体的互补DNA克隆已从小鼠大脑中分离出来，并在非洲爪蟾卵母细胞中对其功能特性进行了研究。该转运体对谷氨酸具有高亲和力（Km = 72微摩尔），且依赖于细胞外的钠离子。cDNA序列预测该蛋白由543个氨基酸组成，具有6个潜在的跨膜结构域。Northern印迹分析表明，编码谷氨酸转运体的mRNA在大脑和外周组织中均有表达。
