Permiakov E A, Deĭkus G Iu
Mol Biol (Mosk). 1995 Jan-Feb;29(1):159-67.
Electron-vibrational spectra of phosphorescence and fluorescence of tryptophan residues in proteins at 77 K are best approximated by theoretical curves computed according to a model which suggests the existence of two independent series of Gaussian vibrational components. Each series contains one type of vibrations. Phosphorescence and fluorescence spectra of proteins with various localizations of their single tryptophan residue were fitted by a curve computed according to this model. The results obtained show that the phosphorescence band of tryptophan residues in proteins seems to contain two types of vibrations with frequencies 650-800 cm-1 and 1350-1500 cm-1. Since the substitution of H2O by D2O does not change the frequencies of both vibrations in the phosphorescence spectra of human serum albumin, melittin and tryptophan in 1 M KCl, it is reasonable to suggest that the 1350-1500 cm-1 series corresponds to the W5 type vibrations (B19a type of vibrations of benzene ring). The 650-800 cm-1 series"can be identified with W18 type of vibrations (breathing vibrations of indole ring). Phosphorescence parameters of tryptophan residues in proteins correlate with their fluorescence parameters.
77K时蛋白质中色氨酸残基的磷光和荧光的电子振动光谱，最好用根据一个模型计算出的理论曲线来近似，该模型表明存在两个独立的高斯振动分量系列。每个系列包含一种类型的振动。用根据该模型计算出的曲线拟合了单个色氨酸残基处于不同位置的蛋白质的磷光和荧光光谱。所得结果表明，蛋白质中色氨酸残基的磷光带似乎包含频率为650 - 800cm-1和1350 - 1500cm-1的两种类型的振动。由于在人血清白蛋白、蜂毒肽和1M KCl中的色氨酸的磷光光谱中，用D2O替代H2O不会改变这两种振动的频率，因此有理由认为1350 - 1500cm-1系列对应于W5型振动（苯环的B19a型振动）。650 - 800cm-1系列可与W18型振动（吲哚环的呼吸振动）相识别。蛋白质中色氨酸残基的磷光参数与其荧光参数相关。
