食物限制延缓了大鼠下丘脑胶质纤维酸性蛋白（GFAP）信使核糖核酸（mRNA）随年龄增长的增加。

Food restriction delays the age-related increase in GFAP mRNA in rat hypothalamus.

作者信息

Nichols N R, Finch C E, Nelson J F

机构信息

Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191, USA.

出版信息

Neurobiol Aging. 1995 Jan-Feb;16(1):105-10. doi: 10.1016/0197-4580(95)80013-h.

DOI:10.1016/0197-4580(95)80013-h

PMID:7723929

Abstract

Astrogliosis with advancing age is correlated with increased expression of glial fibrillary acidic protein (GFAP). Hypothalamic GFAP mRNA prevalence was determined in male F344 rats of different ages that were fed ad lib (AL) and compared with that of rats that were food-restricted (FR) to 60% of AL levels. Hypothalamic GFAP mRNA increased 3-fold at 24 to 25 months in AL rats compared with 3 and 6 month groups. There were no differences in GFAP mRNA levels between AL and FR rats from 3 to 18 months. However, GFAP mRNA was significantly lower in FR than in AL rats at 24 to 25 months; FR rats reached the level of GFAP mRNA in 24 to 25 months AL rats by 33 months. Hypothalamic glutamine synthetase mRNA also increased with age in both dietary groups but did not differ between dietary groups at any age. The observation that FR delays the increased expression of GFAP in the hypothalamus during aging lends support to the hypothesis that upregulation of GFAP mRNA is a biomarker of brain aging.

摘要

随着年龄增长，星形胶质细胞增生与胶质纤维酸性蛋白（GFAP）表达增加相关。测定了自由采食（AL）的不同年龄雄性F344大鼠下丘脑GFAP mRNA的患病率，并与食物限制（FR）至AL水平60%的大鼠进行比较。与3个月和6个月组相比，AL大鼠在24至25个月时下丘脑GFAP mRNA增加了3倍。在3至18个月时，AL大鼠和FR大鼠的GFAP mRNA水平没有差异。然而，在24至25个月时，FR大鼠的GFAP mRNA显著低于AL大鼠；FR大鼠在33个月时达到了24至25个月AL大鼠的GFAP mRNA水平。两个饮食组的下丘脑谷氨酰胺合成酶mRNA也随年龄增加，但在任何年龄饮食组之间没有差异。FR延缓衰老过程中下丘脑GFAP表达增加这一观察结果支持了GFAP mRNA上调是脑衰老生物标志物的假说。