Andres-Barquin P J, Le Prince G, Fages C, Garcia de Jalon J A, Pérez-Martos A, Tardy M, López-Pérez M J
Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.
Mol Chem Neuropathol. 1994 May;22(1):57-65. doi: 10.1007/BF03160094.
Gene expression of two astroglial markers, glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS), was investigated in cerebellum and brainstem from scrapie-affected sheep. The GFAP and GFAP-mRNA concentrations were increased in the two cerebral regions studied in the scrapie-affected animals as compared to the controls. The good correlation between the increase in GFAP and GFAP-mRNA concentrations found in scrapie-affected sheep indicates a significant de novo synthesis of GFAP in this pathology. In contrast to these results, in scrapie no significant differences in GS-mRNA content appeared in either brain area from scrapie-affected sheep as compared to the controls. This fact could suggest some specificity of GFAP expression changes in this pathology. The overexpression of GFAP gene could be related to a possible interaction between GFAP and scrapie infectious agent in astrocytes. The relative increase in the GFAP and its encoding message in affected animals was higher in the cerebellum than in the brainstem, which would suggest regional comparative differences in the effect here described.
在患瘙痒病绵羊的小脑和脑干中，研究了两种星形胶质细胞标志物——胶质纤维酸性蛋白（GFAP）和谷氨酰胺合成酶（GS）的基因表达。与对照组相比，在患瘙痒病动物所研究的两个脑区中，GFAP和GFAP - mRNA浓度均升高。在患瘙痒病绵羊中发现GFAP和GFAP - mRNA浓度的升高之间具有良好的相关性，这表明在这种病理状态下GFAP有显著的从头合成。与这些结果相反，与对照组相比，在患瘙痒病绵羊的任一脑区中，GS - mRNA含量均未出现显著差异。这一事实可能表明在这种病理状态下GFAP表达变化具有一定特异性。GFAP基因的过表达可能与GFAP和瘙痒病感染因子在星形胶质细胞中的可能相互作用有关。在患病动物中，小脑GFAP及其编码信息的相对增加高于脑干，这表明此处所描述的效应存在区域比较差异。
