Suzue T
Department of Physiology, School of Medicine, Tokyo Medical University, Japan.
Neurosci Res. 1994 Dec;21(2):173-6. doi: 10.1016/0168-0102(94)90160-0.
The present study demonstrated that live mouse fetuses in late gestation can be kept in vitro and their physiological activities maintained for over 24 h. Fetuses together with uterus were isolated from pregnant mice at 11.5-17.5 days gestation and transplacentally perfused with a physiological solution through a cannula inserted into the uterine artery. Under these conditions, various physiological activities including heartbeats and spontaneous and stimulation-induced fetal movements were observed.
本研究表明，妊娠晚期的活胎小鼠可在体外保存，其生理活动可维持超过24小时。在妊娠11.5 - 17.5天从怀孕小鼠中分离出带有子宫的胎儿，并通过插入子宫动脉的套管经胎盘灌注生理溶液。在这些条件下，观察到了包括心跳以及自发和刺激诱导的胎儿运动在内的各种生理活动。
