携带核糖体蛋白S20的结构基因rps T的转导噬菌体的分离。
Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20.
作者信息
Friesen J D, Parker J, Watson R J, Fiil N P, Pedersen S
出版信息
Mol Gen Genet. 1976 Feb 27;144(1):115-8. doi: 10.1007/BF00277314.
PMID:772410
Abstract
Lambda transducing phages carrying segments of the Escherichia coli chromosome in the dapB region have been isolated in their in vivo gene products analyzed by two-dimensional gel electrophoresis. One of these phages, lambdaddapB-2, carries the structural genes for ribosomal protein S20 (rps T) and isoleucyltransfer RNA synthetase (ileS.) The most likely gene order is thr-rpsT-ileS-dapB-pyrA.
摘要
携带大肠杆菌染色体dapB区域片段的λ转导噬菌体已被分离出来，并通过二维凝胶电泳分析了它们的体内基因产物。其中一种噬菌体λdapB - 2携带核糖体蛋白S20（rpsT）和异亮氨酰 - 转移RNA合成酶（ileS）的结构基因。最可能的基因顺序是thr - rpsT - ileS - dapB - pyrA。
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