核糖体蛋白S20基因的表达：基因剂量的影响
Expression of the gene for ribosomal protein S20: effects of gene dosage.
作者信息
Parsons G D, Mackie G A
出版信息
J Bacteriol. 1983 Apr;154(1):152-60. doi: 10.1128/jb.154.1.152-160.1983.
Abstract
We have exploited the properties of three different plasmids which carry the gene for Escherichia coli ribosomal protein S20 (rpsT) to test the effects of gene dosage on the expression of rpsT. Over a range of total copies of rpsT of 1 to 58 per haploid genome equivalent, the rate of incorporation of uridine during a 30-s pulse into RNA annealing to either of two specific probes for S20 mRNA increased essentially in proportion to copy number. In contrast, the rate of synthesis of S20 protein increased no more than 2.1-fold at the highest copy number. We conclude, in contrast to an earlier report (D. Geyl, and A. Böck, Mol. Gen. Genet. 154:327-334, 1977), that the synthesis of S20 is regulated at a posttranscriptional step. We propose that S20 itself is the regulatory agent and that binding of S20 to its own mRNA in regions homologous in structure with 16S rRNA can account for our results.
摘要
我们利用了三种携带大肠杆菌核糖体蛋白S20（rpsT）基因的不同质粒的特性，来测试基因剂量对rpsT表达的影响。在每单倍体基因组当量中rpsT总拷贝数为1至58的范围内，在30秒脉冲期间尿苷掺入与S20 mRNA的两种特异性探针之一退火的RNA中的速率基本上与拷贝数成比例增加。相比之下，在最高拷贝数时，S20蛋白的合成速率增加不超过2.1倍。与早期报告（D. Geyl和A. Böck，《分子遗传学与普通遗传学》154:327 - 334，1977）相反，我们得出结论，S20的合成在转录后步骤受到调控。我们提出S20本身就是调节因子，并且S20与其自身mRNA在与16S rRNA结构同源的区域结合可以解释我们的结果。
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