Durbin M L, Learn G H, Huttley G A, Clegg M T
Department of Botany and Plant Sciences, University of California, Riverside 92521-0124, USA.
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3338-42. doi: 10.1073/pnas.92.8.3338.
The evolution of the chalcone synthase [CHS; malonyl-CoA:4-coumaroyl-CoA malonyltransferase (cyclizing), EC 2.3.1.74] multigene family in the genus Ipomoea is explored. Thirteen CHS genes from seven Ipomoea species (family Convolvulaceae) were sequenced--three from genomic clones and the remainder from PCR amplification with primers designed from the 5' flanking region and the end of the 3' coding region of Ipomoea purpurea Roth. Analysis of the data indicates a duplication of CHS that predates the divergence of the Ipomoea species in this study. The Ipomoea CHS genes are among the most rapidly evolving of the CHS genes sequenced to date. The CHS genes in this study are most closely related to the Petunia CHS-B gene, which is also rapidly evolving and highly divergent from the rest of the Petunia CHS sequences.
本文探究了甘薯属中查尔酮合酶[CHS；丙二酰辅酶A：4-香豆酰辅酶A丙二酰基转移酶（环化），EC 2.3.1.74]多基因家族的进化情况。对来自7种甘薯属植物（旋花科）的13个CHS基因进行了测序——3个来自基因组克隆，其余的通过使用根据甘薯Ipomoea purpurea Roth的5'侧翼区域和3'编码区末端设计的引物进行PCR扩增获得。数据分析表明，CHS的一次复制发生在本研究中甘薯属物种分化之前。甘薯属的CHS基因是迄今为止测序的CHS基因中进化最快的基因之一。本研究中的CHS基因与矮牵牛CHS-B基因关系最为密切，矮牵牛CHS-B基因也在快速进化，并且与矮牵牛其他CHS序列高度不同。
