Fukasawa K, Vande Woude G F
Advanced BioScience Laboratories-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702, USA.
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3430-4. doi: 10.1073/pnas.92.8.3430.
High levels of mos protooncogene product are expressed during oocyte meiotic maturation and Mos has been implicated in formation of the spindle and spindle pole. Here, we show that in Swiss 3T3 cells with 4N DNA content, high levels of Mos lead to the production of binucleated cells. The Swiss 3T3 cells in mitosis, before binucleation occurs, are anastral and the spindle poles are juxtaposed to the cell membrane. These phenotypes may be related to the meiotic process of attachment of the spindle pole to the oocyte membrane during polar body formation. The production of binucleated somatic cells could result from attachment of the altered mitotic spindle pole to the cell membrane that interferes with cytokinesis but not karyokinesis. This can explain at least one form of genetic instability that leads to altered DNA content in tumor cells.
在卵母细胞减数分裂成熟过程中会表达高水平的mos原癌基因产物，并且Mos与纺锤体和纺锤极的形成有关。在此，我们表明在具有4N DNA含量的瑞士3T3细胞中，高水平的Mos会导致双核细胞的产生。在双核形成之前处于有丝分裂期的瑞士3T3细胞无星纺锤体，且纺锤极与细胞膜并列。这些表型可能与极体形成过程中纺锤极附着于卵母细胞膜的减数分裂过程有关。双核体细胞的产生可能是由于改变的有丝分裂纺锤极附着于细胞膜，从而干扰胞质分裂而非核分裂所致。这至少可以解释导致肿瘤细胞DNA含量改变的一种遗传不稳定性形式。
