Paranos S, Bojović S, Miletić V, Despotović N
Department of Allergy and Clinical Immunology, Zvezdara University Hospital, Belgrade.
Srp Arh Celok Lek. 1993 Aug-Dec;121(8-12):120-3.
Intradermal injection with alpha-lactoalbumine, 2000 PNU/ml was carried out in seven patients (1 male, 6 females, mean age 32.4 years) suffering from milk intolerance. None of the patients had a positive allergen-specific IgE antibodies in the sera (RAST). Skin test was performed on the volar part of the forearms at the same time. The area of skin reaction was estimated and measured within 36 hours on the right arm. Skin biopsy was done with a 4 mm punch after 8 hours from site of antigen injection on the left arm. Various cells infiltrating the site of antigen instillation were present. Leukocytoclastic vasculitis was only found in patients with dual intradermal reaction (immediate and late onset of response; 8 12 hours). These results indicate that local skin reaction to alpha-lactoal-bumine is mediated by diverse immune mechanisms, and that it may be of clinical significance for the diagnosis of cow milk allergy in adults.
对7名患有牛奶不耐受症的患者（1名男性，6名女性，平均年龄32.4岁）进行了皮内注射2000PNU/ml的α-乳白蛋白。所有患者血清中均无阳性变应原特异性IgE抗体（放射性变应原吸附试验）。同时在前臂掌侧进行皮肤试验。在36小时内对右臂皮肤反应面积进行估计和测量。在左臂抗原注射部位8小时后，用4毫米打孔器进行皮肤活检。抗原注入部位有各种细胞浸润。白细胞破碎性血管炎仅在有双重皮内反应（即时和迟发性反应；8至12小时）的患者中发现。这些结果表明，局部皮肤对α-乳白蛋白的反应是由多种免疫机制介导的，并且对于成人牛奶过敏的诊断可能具有临床意义。
