Uni Z, Pratt W D, Miller M M, O'Connell P H, Schat K A
Department of Avian and Aquatic Animal Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Vet Immunol Immunopathol. 1994 Dec;44(1):57-69. doi: 10.1016/0165-2427(94)90169-4.
Cell-mediated immune responses against Marek's disease virus (MDV) antigens were examined using reticuloendotheliosis virus (REV)-transformed cell lines of two haplotypes (B19B19 and B13B13). These cell lines were stably transfected with cloned fragments of MDV DNA resulting in the expression of the MDV-specific phosphoprotein pp38. Effector cells were obtained from P2a (B19B19) and S13 (B13B13) chickens at 7 days post inoculation with REV, oncogenic or attenuated serotype 1 MDV (JM-16/O and JM-16/A, respectively), serotype 2 MDV (SB-1), or herpesvirus of turkeys (HVT). Transfection of MDV genes did not influence the expression of Class I major histocompatibility complex antigens. The optimal effector to target cell ratio was determined to be 100:1. REV-sensitized effector cells lysed REV cell lines and REV cell lines transfected with MDV DNA in a syngeneic fashion. Effector cells from chickens inoculated with JM-16/O, JM-16/A, SB-1 or HVT lysed only the syngeneic, transfected cell lines, but not the parent REV cell lines. The percentage specific release caused by the MDV-sensitized effector cells was low, but statistically significant.
利用两种单倍型（B19B19和B13B13）的网状内皮组织增殖病病毒（REV）转化细胞系，检测了针对马立克氏病病毒（MDV）抗原的细胞介导免疫反应。这些细胞系用MDV DNA克隆片段进行稳定转染，导致MDV特异性磷蛋白pp38的表达。效应细胞在接种致癌或减毒1型MDV（分别为JM - 16/O和JM - 16/A）、2型MDV（SB - 1）或火鸡疱疹病毒（HVT）的REV后7天，从P2a（B19B19）和S13（B13B13）鸡中获得。MDV基因的转染不影响I类主要组织相容性复合体抗原的表达。确定最佳效应细胞与靶细胞比例为100:1。REV致敏的效应细胞以同基因方式裂解REV细胞系和用MDV DNA转染的REV细胞系。接种JM - 16/O、JM - 16/A、SB - 1或HVT的鸡的效应细胞仅裂解同基因的、转染的细胞系，而不裂解亲本REV细胞系。MDV致敏的效应细胞引起的特异性释放百分比很低，但具有统计学意义。
