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针对马立克氏病病毒(MDV)即刻早期、晚期和独特蛋白的同基因MDV特异性细胞介导免疫反应。

Syngeneic Marek's disease virus (MDV)-specific cell-mediated immune responses against immediate early, late, and unique MDV proteins.

作者信息

Omar A R, Schat K A

机构信息

Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.

出版信息

Virology. 1996 Aug 1;222(1):87-99. doi: 10.1006/viro.1996.0400.

Abstract

Marek's disease (MD) infection has been controlled effectively by vaccination using nononcogenic and/or attenuated oncogenic Marek's disease virus (MDV) vaccines. Thus far, there is little knowledge on the role of cell-mediated immune (CMI) responses during MDV infection or vaccination. To elucidate the importance of MDV proteins in CMI responses, the pp38, Meq, ICP4, or ICP22 genes of an oncogenic strain, GA and the gB, ORF A, A41, or L1 genes of a highly oncogenic strain, RB1B were stably transfected into reticuloendotheliosis virus (REV)-transformed lymphoblastoid cells, CU-91 (MHC: B19B19) and CU-205 (MHC: B21B21). Cell lines positive for MDV gene transcription and/or protein expression were used in a standard 4-hr chromium release assay. Effector cells for this assay were obtained from splenocytes of chickens infected with the oncogenic strain, JM-16/13 or the nononcogenic vaccine strain, SB-1/12. Cell lines expressing MDV pp38, Meq, or gB were lysed by syngeneic but not allogeneic MDV-sensitized splenocytes obtained from chickens of B19B19 and B21B21 haplotypes. However, syngeneic CMI responses against ICP4 were detected only in B21B21 chickens. CMI responses were not detected against B19B19 and B21B21 cell lines expressing A41, L1, ORF A, or ICP22. This report suggests that syngeneic CMI responses against pp38, Meq, ICP4, and gB of GA and RB1B strains, respectively, can be induced in chickens inoculated with JM16/13 or SB-1/12. The difference in CMI response to ICP4 in genetically susceptible (B19B19) and genetically resistant (B21B21) chickens may be an important factor in genetic resistance.

摘要

通过使用非致瘤性和/或减毒致瘤性马立克氏病病毒(MDV)疫苗进行接种,马立克氏病(MD)感染已得到有效控制。迄今为止,关于细胞介导免疫(CMI)反应在MDV感染或接种过程中的作用知之甚少。为了阐明MDV蛋白在CMI反应中的重要性,将致瘤性毒株GA的pp38、Meq、ICP4或ICP22基因以及高致瘤性毒株RB1B的gB、ORF A、A41或L1基因稳定转染到网状内皮组织增生症病毒(REV)转化的淋巴母细胞系CU-91(MHC:B19B19)和CU-205(MHC:B21B21)中。MDV基因转录和/或蛋白表达呈阳性的细胞系用于标准的4小时铬释放试验。该试验的效应细胞取自感染致瘤性毒株JM-16/13或非致瘤性疫苗毒株SB-1/12的鸡的脾细胞。表达MDV pp38、Meq或gB的细胞系被来自B19B19和B21B21单倍型鸡的同基因但非异基因MDV致敏脾细胞裂解。然而,仅在B21B21鸡中检测到针对ICP4的同基因CMI反应。未检测到针对表达A41、L1、ORF A或ICP22的B19B19和B21B21细胞系的CMI反应。本报告表明,接种JM16/13或SB-1/12的鸡分别可诱导针对GA和RB1B毒株的pp38、Meq、ICP4和gB的同基因CMI反应。遗传易感(B19B19)和遗传抗性(B21B21)鸡对ICP4的CMI反应差异可能是遗传抗性的一个重要因素。

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