Bryon P A
Pathol Biol (Paris). 1976 Jan;24(1):75-9.
A routine method of epoxy embedding of bone-marrow and other tissues for light microscopy is described. Undecalcified bone specimens obtained from the sternum or iliac crest using trephine biopsy are fixed in formalin, deshydrated in acetone, embedded in a Kushida's low-viscosity epoxy resin medium. The samples are cut using a carbide-edged knife in a rotary microtome or a glass knife in a Sorval JB4 microtome. After fixing sections to slide with Neoprene and removal of the embedding medium with sodium methoxide and acetone, the staining with usual histological procedures is quite easy. A simple and rapid technique is outlined for staining these sections with Giemsa. The resolution, compared with routine parafin sections, is improved.
本文描述了一种用于光学显微镜检查的骨髓及其他组织环氧包埋常规方法。使用环钻活检从胸骨或髂嵴获取的未脱钙骨标本，用福尔马林固定，丙酮脱水，嵌入久志田低粘度环氧树脂介质中。样品在旋转切片机上用硬质合金刀片或在索瓦JB4切片机上用玻璃刀片切割。用氯丁橡胶将切片固定在载玻片上，并用甲醇钠和丙酮去除包埋介质后，采用常规组织学方法染色相当容易。概述了一种用吉姆萨染色这些切片的简单快速技术。与常规石蜡切片相比，分辨率有所提高。
