Griffin A M, Morris V J, Gasson M J
Institute of Food Research, Norwich Laboratory, Colney, UK.
Int J Biol Macromol. 1994 Dec;16(6):287-9. doi: 10.1016/0141-8130(94)90057-4.
We have identified, cloned and sequenced an 8422 base pair fragment of Acetobacter xylinum genomic DNA containing part of the acetan biosynthetic gene cluster. Computer analysis of the nucleotide sequence data generated revealed the presence of six open reading frames. Comparison of the translated sequences of putative genes to the amino acid sequences of genes from other organisms was used to assign functions to the aceA, aceC and manB genes. These genes were predicted to encode a UDP-glycosyl transferase, a GDP-mannosyl transferase and a phosphomannose isomerase/GDP-mannose pyrophosphorylase, respectively.
我们已经鉴定、克隆并测序了木醋杆菌基因组DNA的一个8422碱基对片段，该片段包含乙酰聚糖生物合成基因簇的一部分。对所产生的核苷酸序列数据进行计算机分析后发现存在六个开放阅读框。通过将推定基因的翻译序列与其他生物体基因的氨基酸序列进行比较，来确定aceA、aceC和manB基因的功能。预计这些基因分别编码一种UDP-糖基转移酶、一种GDP-甘露糖基转移酶和一种磷酸甘露糖异构酶/GDP-甘露糖焦磷酸化酶。
