木醋杆菌中参与乙偶姻生物合成的aceA基因的鉴定、克隆及测序
Identification, cloning and sequencing the aceA gene involved in acetan biosynthesis in Acetobacter xylinum.
作者信息
Griffin A M, Morris V J, Gasson M J
机构信息
Institute of Food Research, Norwich Laboratory, Colney, UK. annette.griffin/b
出版信息
FEMS Microbiol Lett. 1996 Mar 15;137(1):115-21. doi: 10.1111/j.1574-6968.1996.tb08092.x.
The aceA gene from Acetobacter xylinum was identified and cloned from a genomic DNA library. The complete DNA sequence was determined and computer analysis of the translated gene sequence revealed homology with the deduced amino acid sequence of gumD from Xanthomonas campestris. Therefore aceA is likely to encode the phosphate-prenyl glucose l-phosphate transferase catalyzing the first step in acetan biosynthesis in A. xylinum.
从木醋杆菌的基因组DNA文库中鉴定并克隆了aceA基因。测定了其完整的DNA序列，对翻译后的基因序列进行计算机分析后发现，它与野油菜黄单胞菌中gumD推导的氨基酸序列具有同源性。因此，aceA可能编码磷酸异戊烯基葡萄糖1-磷酸转移酶，该酶催化木醋杆菌中乙酰聚糖生物合成的第一步反应。
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